Tomato (Solanum lycopersicum L.) is a major fruit crop of Solanaceae family. It is consumd worldwide and has been widely used as a model plant system for understanding biological processes, functional genomics, proteomics, and metabolomics. In vitro culture, a major plant biotechnology tool, exploits the totipotent nature of plant cells In recent times, genetic transformation and genome editing of plants using plant tissue and cell culture have become an important tool in both basic and applied research by insertion or deletion of target DNA. There are several factors which affect the tissue culture and regeneration of tomato. Here, we have discussed the effect of genotype; explant type, effect of age, size and orientation of explants, effect of light and temperature, nutrient media, sugar concentration, and plant growth regulators on the regeneration of tomato.